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Decryption of analyzes

Analysis of feces for dysbiosis: decoding what it is and how to properly pass?

~ 3 kg of bacteria inhabit human intestines. They represent the microflora necessary for normal digestion, but with various disruptions the number of some microorganisms can decrease markedly - dysbacteriosis will occur - an imbalance of bacteria.

Although doctors do not attribute it to independent diseases, the harm from this does not diminish. If you suspect an intestinal dysbacteriosis, special tests of feces are prescribed to establish an accurate diagnosis.


Analysis of feces for dysbiosis, what is it?

Analysis of feces for dysbiosis, what is it?

Microorganisms that live in the digestive tract, are of paramount importance for human life. They synthesize vitamins, break down food and protect against attacks of pathogenic strains.

In other words, man and bacteria are in symbiosis. But in violation of the composition of the microflora, the occurrence of flatulence, diarrhea, nausea, not to mention the consequences of insufficient intake of nutrients in the tissue.

The purpose of stool analysis is to determine the qualitative and quantitative composition of bacteria in the intestine.

To do this, in medicine usually use 3 methods:

  1. Coprogram.
  2. Bacteriological analysis.
  3. Biochemical analysis.


Coprogram is prescribed for treating a person with complaints of chronic or acute disorder of the stool, abdominal pain of incomprehensible nature, a sharp weight loss for no apparent reason.

Doctors also resort to such research at treatment of the diseases which are not connected with a gastrointestinal tract. This is especially true with antibiotic therapy pathologies in different parts of the body (throat, joints, etc.).

A coprogram is a primary examination, which is only an auxiliary method and gives a physical characteristic to the contents of the intestine.

The analysis is carried out in 2 stages:

1. Macroscopic:

  • color of feces;
  • the form;
  • quantity;
  • smell;
  • the presence of parasites;
  • the presence of pus, blood and mucus;
  • leftover uncooked food.

2. Microscopic:

  • cells and tissue fragments;
  • digested food (fiber, fat, salt, starch, etc.).

Bacteriological analysis of feces

Bacteriological analysis of feces If the coprogram demonstrates deviations from the norm, the doctor has a reason to conduct a more thorough analysis. In the laboratory, perform a culture of feces on a nutrient medium.

After 4-5 days, the bacteria multiply, which will allow to examine their colonies under a microscope. After that, the specialist makes a conclusion about the number of microbes in 1 g of feces (CFU / g).

Based on the data, the doctor makes a diagnosis. The results of analyzes of adults and children often differ, so the age of the patient is necessarily taken into account.

But the 5-day expectation of the growth of colonies is not always permissible, because during this time a person's condition may deteriorate significantly.

Biochemical analysis of feces

Biochemical analysis of feces for dysbacteriosis gives the result on the day of sampling. The essence of this study is to identify compounds present in the intestine.

Particular attention is paid to the spectrum of fatty acids, because they are synthesized by bacteria in the process of life. Another biochemical analysis is called rapid diagnosis.

The method is very informative and simple, it not only demonstrates the imbalance of microflora, but also establishes the section of the intestine in which the malfunction occurred.

Doctors are much more likely to give preference to this study due to its significant advantages:

  • Speed. Results will be in 1-2 hours.
  • Sensitivity. The method very accurately determines the concentration of compounds.
  • Undemanding to freshness samples. Even yesterday's feces will do.

Preparation for analysis of feces

Preparation for analysis of feces The reliability of the research results depends on the correct preparation. The fact is that many foods contain substances that will give a positive reaction.

First of all, it is meat. Hemoglobin is present in it.

Secondly, it is iron. All red products have this element in their composition. It is necessary to refrain from the use of such dishes for 3 days before the analysis, so that the laboratory does not accidentally get a false positive result.

Restrictions apply to raw vegetables and fruits: during the preparation period, only heat-treated vegetable products should be eaten.

In addition, the patient should stop taking medications that directly affect the intestinal microflora:

  • antibiotics;
  • probiotics;
  • laxatives (official and popular);
  • rectal suppositories.

Preparations for the analysis of feces for dysbiosis adults perform their own. Examination of the contents of the children's intestines is no different, but parents will have to monitor compliance with all the recommendations of the child.

How to properly pass stool analysis for dysbacteriosis?

Diet and drug withdrawal are the primary conditions for the reliability of the analysis results. In addition, the patient will need to collect feces in accordance with the rules.

We hand over cal - 6 rules:

  1. Wash the perineum before the control defecation (the probability of ingress of old samples is excluded).
  2. It is forbidden to use any aids to speed up the process of defecation (enema, laxative).
  3. Prepare in advance a special container with a tight lid (must be purchased at the pharmacy).
  4. Do not allow the ingress of fluid into the feces (urine, water, etc.).
  5. Take 3 fragments of feces (1 teaspoon from different sites).
  6. If there is blood or mucus, then such samples are taken without fail.

Intestinal bacteria are mostly anaerobic. After 1 hour after defecation, they will still retain their population in their natural form, but gradually the microorganisms will begin to die.

In order to properly pass fecal dysbacteriosis analysis, it is necessary to deliver samples of excreta to the laboratory at least 2 hours after emptying.

Urgency is not so essential for biochemical research, which studies not the colonies of bacteria, but the result of their vital activity - fatty acids. These compounds spontaneously almost do not decay, and therefore remain unchanged for a long time.

Doctors can even freeze the feces and bring it the next day. In the case of newborn children, this option is sometimes the most preferable for parents.

Deciphering the results of the analysis of feces for dysbacteriosis

Deciphering the results of the analysis of feces for dysbacteriosis

There are 100 trillion bacteria in the intestines, which is 10 times the number of all cells in the body. If the microbes are not at all, then the person will simply die.

On the other hand, a shift in balance in any direction leads to diseases. Decoding analysis of feces for dysbacteriosis is to determine the number and types of microbes.

Table of interpretation of results and standards of analysis

Children under 1 year Older children Adults
Bifidobacteria 10 10 - 10 11 10 9 - 10 10 10 8 - 10 10
Lactobacillus 10 6 - 10 7 10 7 - 10 8 10 6 - 10 8
Escherichia 10 6 - 10 7 10 7 - 10 8 10 6 - 10 8
Bacteroids 10 7 - 10 8 10 7 - 10 8 10 7 - 10 8
Peptostreptokokki 10 3 - 10 5 10 5 - 10 6 10 5 - 10 6
Enterococci 10 5 - 10 7 10 5 - 10 8 10 5 - 10 8
Saprophytic Staphylococcus ≤10 4 ≤10 4 ≤10 4
Pathogenic Staphylococcus - - -
Clostridia ≤10 3 ≤10 5 ≤10 5
Candida ≤10 3 ≤10 4 ≤10 4
Pathogenic enterobacteria - - -

Detailed decoding:

1. Bifidobacteria:

  • 95% of all bacteria found in the intestines;
  • synthesize vitamins K and B;
  • promote the absorption of vitamin D and calcium;
  • strengthen the immune system.

2. Lactobacilli:

  • maintain acidity;
  • synthesize lactase and protective substances.

3. Escherichia:

  • synthesize vitamins K and B;
  • promote the absorption of sugars;
  • produce colicins - proteins that kill germs.

4. Bacteroids:

  • break down fats;
  • perform a protective function.

5. Streptococci:

  • break down carbohydrates;
  • perform a protective function;
  • present in small quantities and not always.

6. Enterococci:

  • break down carbohydrates.

7. Peptococci:

  • participate in the synthesis of fatty acids;
  • perform a protective function;
  • not always present.

8. Staphylococcus:

  • inhabit the large intestine;
  • participate in nitrate exchange;
  • There are many pathogenic strains.

9. Clostridia:

  • inhabit the large intestine;
  • synthesize acids and alcohols;
  • break down proteins.

10. Fungal:

  • maintain an acidic environment;
  • conditionally pathogenic.

A change in the number of certain microorganisms is possible if the disease strains enter the intestine.

This usually occurs when personal hygiene is not observed (dirty hands, unwashed fruits and vegetables). Antibiotic treatment is the second most common cause of dysbiosis.

To normalize the situation in the gastrointestinal tract, doctors additionally prescribe probiotics - special dietary supplements.

In addition, dysbacteriosis often indicates an immune failure. Leukocytes control the microbial population, the number of which increases significantly as the level of natural protection decreases. And often not useful bacteria, and pathogenic.

Analysis of feces in children

The results of the analysis of feces for dysbiosis in children are somewhat different than in adults. This is due, primarily, with the gradual colonization of the intestines by microorganisms.

After birth, the baby eats mother's milk, which contributes to the development of normal microflora. But in hospitals, infection with Staphylococcus aureus often occurs.

And if the mother does not have antibodies to this microorganism, then the baby will earn dysbiosis.

In addition, some useful strains appear only for 1 year, for example, bacteroids. Sometimes in the intestines of a child, fungi of the genus Candida develop excessively, which provoke the corresponding disease - candidiasis.

The most common cause of dysbiosis in children is the early transition to artificial feeding. Still, the baby needs mother's milk in the first year of life.


The analysis of feces for dysbacteriosis is prescribed for any digestive disorders. In addition, doctors monitor the state of the patient's microflora during antibiotic therapy.

Timely detection of dysbiosis and clarification of the nature of the disorder will provide an opportunity to take the right steps and reduce the likelihood of complications.


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